Fig. 4

14-3-3 inhibition increases αsyn inclusion formation. a Representative images of eYFP-difopein immunostaining in the cortex of WT and difopein (“cortical” line 138) mice at 3 mpi. GFP-difopein expression is found only in difopein mice. Scale bar = 100 μm. b Representative images of pS129-αsyn immunostaining in the cortex of WT and difopein mice at 3 mpi. Scale bar = 100 μm. c Difopein expression in the cortex increases inclusion counts at 3 mpi and decreases counts at 6 mpi in the cortex. Quantification of pS129-αsyn positive inclusions at 3 mpi and 6 mpi in the cortex of PFF-injected WT or difopein mice. n = 15–16 per group at 3mpi; n = 13–14 per group at 6mpi. *p < 0.05 (Student’s t-test). Error bars represent SEM. d Representative images of eYFP-difopein immunostaining in the SN of WT and difopein (“nigral” line 166) mice at 3 mpi. GFP-difopein expression is found only in difopein mice. Scale bar = 100 μm. e Representative images of pS129-αsyn immunostaining in the SN of WT and difopein mice at 3 mpi. Scale bar = 100 μm. f Difopein expression in in the nigra increases inclusion counts at 3 mpi and decreases counts at 6mpi in the SN. Quantification of pS129-αsyn positive inclusions at 3 mpi and 6 mpi in the SN of PFF-injected WT and difopein mice. n = 7–9 per group at 3 mpi; n = 10–13 per group at 6 mpi. *p < 0.05 (Student’s t-test). Error bars represent SEM