Type 2 diabetes mellitus-associated transcriptome alterations in cortical neurones and associated neurovascular unit cells in the ageing brain

Table 2 Antibody source and specificity

Antibody	Isotype	Dilution (time, temp)	Antigen retrieval	Supplier
AGE	Rabbit IgG	1:2000 (60 min, RT)	MW 10 min, TSC	Abcam, UK
COL4	Rabbit IgG	1:200 (3 min, RT)	N/A	Abcam, UK
COX5b	Rabbit IgG	1:50 (60 min, RT)	MW 10 min, EDTA	Thermo Fisher Scientific, UK
FOXO3a	Rabbit IgG	1:100 (O/N, 4 °C)	PC, TSC	Abcam, UK
GFAP	Rabbit IgG	1:50 (3 min, RT)	N/A	Dako
γH2AX	Rabbit IgG	1:2000 (60 min, RT)	PC, EDTA	R&D Systems, UK
IGF-1Rβ	Rabbit IgG	1:25 (60 min, RT)	N/A	Santa Cruz Biotechnology, UK
NDUFb6	Rabbit IgG	1:100 (60 min, RT)	MW 10 min, EDTA	Sigma-Aldrich, UK
p53	Mouse IgG	1:50 (O/N, 4 °C)	PC, MenaPath Access Super	Santa Cruz Biotechnology, UK
TGFβ1	Rabbit IgG	1:400 (60 min, RT)	PC, EDTA	Abcam, UK

AGE, advanced glycation end-product; COL4, collagen IV; COX5b, cytochrome C oxidase subunit 5B; EDTA, ethylenediaminetetraacetic acid; FOXO3a, forkhead box O3; GFAP, glial fibrillary acid protein; IGF-1Rβ, insulin-like growth factor 1 receptor beta; IgG, immunoglobulin G; MW, microwave; N/A, not applicable (frozen tissue); NDUFb6, NADH:ubiquinone oxidoreductase subunit B6; O/N, overnight; p53, tumour protein p53; PC, pressure cooker; RT, room temperature; TB, toluidine blue; TGFβ1, transforming growth factor beta 1; TSC, trisodium citrate; γH2AX, gamma H2A histone family member X

ISSN: 2051-5960