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Table 2 All antibodies used with information on species, isotype, class, catalogue number and working dilution. Figure shows antibody combinations used for (a) α-sarcoglycan (b) β-dystroglycan and (c) f/d myosin triple stains

From: The administration of antisense oligonucleotide golodirsen reduces pathological regeneration in patients with Duchenne muscular dystrophy

Primary antibody Species Class Catalogue # Dilutions
β-dystroglycan Mouse IgG2a Monoclonal NCL-b-DG 1:20
Dystrophin (exon 77) Rabbit IgG Polyclonal ab15277 1:200
Laminin α2-1 (300 kDa) Rat IgG1 Monoclonal 804-190-C100 1:50
Myosin developmental Mouse IgG1 Monoclonal NCL-MHCd 1:30
Myosin neonatal Mouse IgG1 Monoclonal NCL-MHCn 1:30
α-sarcoglycan Mouse IgG1 Monoclonal NCL-a-SARC 1:40
Secondary antibody Species Class Catalogue # Dilutions
Alexa Fluor 488 Goat anti-Rabbit IgG Polyclonal A-11034 1:100
Alexa Fluor 568 Goat anti-Rat IgG Polyclonal A-11077 1:100
Alexa Fluor 647 Donkey anti-Mouse IgG Polyclonal A-31571 1:100