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Table 2 All antibodies used with information on species, isotype, class, catalogue number and working dilution. Figure shows antibody combinations used for (a) α-sarcoglycan (b) β-dystroglycan and (c) f/d myosin triple stains

From: The administration of antisense oligonucleotide golodirsen reduces pathological regeneration in patients with Duchenne muscular dystrophy

Primary antibody

Species

Class

Catalogue #

Dilutions

β-dystroglycan

Mouse IgG2a

Monoclonal

NCL-b-DG

1:20

Dystrophin (exon 77)

Rabbit IgG

Polyclonal

ab15277

1:200

Laminin α2-1 (300 kDa)

Rat IgG1

Monoclonal

804-190-C100

1:50

Myosin developmental

Mouse IgG1

Monoclonal

NCL-MHCd

1:30

Myosin neonatal

Mouse IgG1

Monoclonal

NCL-MHCn

1:30

α-sarcoglycan

Mouse IgG1

Monoclonal

NCL-a-SARC

1:40

Secondary antibody

Species

Class

Catalogue #

Dilutions

Alexa Fluor 488

Goat anti-Rabbit IgG

Polyclonal

A-11034

1:100

Alexa Fluor 568

Goat anti-Rat IgG

Polyclonal

A-11077

1:100

Alexa Fluor 647

Donkey anti-Mouse IgG

Polyclonal

A-31571

1:100

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