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Table 2 Primary antibodies II (rat tissue)

From: Lack of astrocytes hinders parenchymal oligodendrocyte precursor cells from reaching a myelinating state in osmolyte-induced demyelination

Antibody

Species

Dilution

Antigen retrieval

Manufacturer

Anti-BCAS1 (Anti-NABC1)

Rabbit

1:100

Citrate, MW

Abcam, ab106661

Anti-BrdU

Mouse

1:400

Citrate, MW

Millipore, MAB3424

Anti-CD68 (clone ED1)

Mouse

1:500

Citrate, MW

Biorad, MCA341R

Anti-GFAP

Rat

1:1000

Citrate, MW

Thermo Fisher, Z0334

Anti-Ki67

Rabbit

1:200

Citrate, MW

DCS, K1681C01

Anti-MAG

Mouse

1:1000

Citrate, MW

Abcam, ab89780

Anti-MBP

Rabbit

1:500

–

Dako, A0623

Anti-Nestin

Mouse

1:50

Citrate, MW

Abcam, ab6142

Anti-NG2

Rabbit

1:200

EDTA, MW

Millipore, AB5320

Anti-Olig2

Mouse

1:100

EDTA, MW

Millipore, MABN50

Anti-Olig2

Rabbit

1:50

EDTA, MW

IBL, 18953

Anti-TPPP/p25

Rabbit

1:500

EDTA, MW

Abcam, ab92305

Anti-PDGFRalpha

Rabbit

1:100

Citrate, MW

Abcam, ab203491