Fig. 2

Mature oligodendrocytes reappear 2 weeks, myelin 3 weeks after lesion initiation. a Mature oligodendrocytes (TPPP/p25; white; arrowheads) and CD68+ activated microglia/macrophages (purple) at different time points after lesion induction. Nuclei are stained in blue (DAPI); original magnification × 200, scale bar 50 µm. b Quantification of TPPP/p25+ mature oligodendrocyte densities at different time points after correction of serum sodium levels (white bars = lesion, grey bars = perilesion, light grey bar = controls; one dot represents one animal, mean ± SEM, one-way ANOVA and Tukey’s multiple comparison, *p < 0.05, **p < 0.01, ***p < 0.001). c Representative ODS lesion at 21 dpc. LFB/PAS staining as well as anti-MAG and anti-PLP immunohistochemistry are shown. Dashed line marks the lesion border. Magnification × 40, scale bar 200 µm