Fig. 8
From: Amyloid β and tau pathology in brains of aged pinniped species (sea lion, seal, and walrus)
Immunohistochemistry for GSK-3β in pinniped brains (case No. 9). The cytoplasmic uniform distribution of GSK-3β and GSK-3β-positive grains was found in the neuronal somata of aged pinnipeds (a). P-GSK-3β (Tyr216)-positive cells were found in aged pinniped brains (b). Double-labeling immunofluorescence in the cerebral cortex of aged pinnipeds revealed that p-GSK-3β (Tyr216)-positive grains were localized in AT8-positive cells (c) Reactions to AT8 are shown in red, and those to GSK-3β and p-GSK-3β (Tyr216) in green. Scale bars: 20 μm (a, b); 10 μm (c). Western blotting analysis of GSK-3β in TBS-soluble fractions from the forebrain of pinnipeds. A distinct band of GSK-3β with a molecular weight of 46 kDa was detected in pinniped brains (arrow, d). A stronger band of p-GSK-3β (Tyr216) was detected in case No. 8 and 9, in which severe hp-tau accumulation was confirmed immunohistochemically, than in other cases (arrow, e)