Fig. 2
From: Amyloid β and tau pathology in brains of aged pinniped species (sea lion, seal, and walrus)
Amyloid plaques in the cerebral cortex of aged pinnipeds (case No. 9). PAM-positive amyloid plaques were observed in the parenchyma of the cerebral cortex (a). Amyloid plaques were morphologically classified into two types, small round plaques (b) and large plaques (c), and were positive for Gallyas-Braak silver staining (d). Immunohistochemically, amyloid plaques were positive for Aβ42 (e, f), Aβ40 (g), AβN1 (h), AβpN3 (i), and ApoE (j). The moderate infiltration of GFAP-positive astrocytes (k) and Iba-1-positive microglia (l) was observed around and on Aβ42-positive amyloid plaques. Swollen dystrophic neurites (arrows) were observed and accompanied by Aβ42-positive amyloid plaques (m). Reactions to Aβ42 (k, l, m) are shown in brown, and those to GFAP (k), Iba-1 (l), and synaptophysin (m) in red. Scale bars: 100 μm (a); 20 μm (b–d, f–k, m); 50 μm (l); 200 μm (e)