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Fig. 7 | Acta Neuropathologica Communications

Fig. 7

From: Mitochondrial defects in the respiratory complex I contribute to impaired translational initiation via ROS and energy homeostasis in SMA motor neurons

Fig. 7

SMN levels are regulated by pyruvate and ROS modifying drugs. a–d Representative western blot images and quantification of SMN levels after pyruvate treatment for 1 h in (a) WT MNs and (b) SMA MNs or after ROS modification in (c) WT MNs and (d) SMA MNs. e 100 nM WYE-687 dihydrochloride blocks elevation of SMN levels after pyruvate treatment in WT MNs. f–h Representative western blot images and quantification of phosphorylated S6 and total S6 protein levels after pyruvate treatment in (f) WT MNs (N = 7) and (g) SMA MNs (N = 7) or after (h) NAC and menadione treatment in SMA MNs (N = 4). One-way ANOVA with Dunnett post hoc analysis was used to compare each time point with the control (a, b). One-way ANOVA with Tukey HSD post hoc analysis was used to determine statistical significance for multiple comparisons (c-h). Each dot represents the quantification of individual biological replicates. Bar graphs depict the mean ± S.D., n.s. p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001

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