Fig. 4

The pericyte characteristics of FAP+ cells in mouse glioblastoma. a, b Double immunohistochemistry (IHC) of FAP (red)/nestin (blue) (none were detected) (a) and FAP (red)/PDGFRβ (blue) (b) in mouse glioblastoma, 005 and GL261. Red arrows point to representative double positive cells. c Animal experiment scheme (top) and flow cytometry analysis (bottom) of the distribution of FAP+ and GFP+ 005 cells. d qRT-PCR analysis of TGF-β1 (Tgfb1) RNA levels in FAP+ and FAP- cells after sorting with flow cytometry. **p < 0.01 (t test). e Double immunofluorescence of FAP (green) and TGF-β1 (red) in mouse glioblastoma. a, b, e Quantification plots on the right. Error bars, SD