Fig. 3

Single cell RNA sequencing analysis identifies FAP^high/PDGFRB^high cells as non-endothelial vascular cells in GBM. a Mapping of FAP and pericyte markers onto tSNE cell clusters of single cell RNAseq analysis of GBM. Plots show RNA levels of different cell types. b Correlation analysis of FAP and PDGFRB transcript levels identifies a subset of cells that co-express FAP and PDGFRB at high levels (red circle). R, Pearson’s R. c Annotation of the 18 clusters identified by a new tSNE analysis with the 12 distinct cell types described by Darmanis et al. d Integration of FAP and PDGFRB expression levels into the new tSNE map to determine the phenotype of FAP^high/PDGFRB^high cells