Fig. 6
Hippocampal dendritic spine loss correlates with RGC dendritic changes in Tg2576, 3xTg-AD and APPNL-G-F mice. a Representative confocal images of DiI labelled dendritic segments of CA1 segments from WT Control, Tg2576, B6J Control, 3xTg-AD and APPNL-G-F. Scale bar 5 µm. Spine densities were analysed from dendritic segments of at least 30 µm b Overall spine density. c Stubby spine density. d Mushroom spine density. e Thin spine density. f Total number of dendritic spines compared to RGC AUC per mouse for 3xTg-AD and APPNL-G-F models (Spearman correlation). g Total number of thin dendritic spines compare to RGC AUC per mouse for 3xTg-AD and APPNL-G-F models (Spearman correlation). Data represented as average per mouse. g Column Scatterplot data points represent individual cells and error bars correspond to SD. Overall (h), stubby (i), mushroom (j) and thin (k) spine density from B6/J Control, 3xTg-AD and APPNL-G-F mice at 6-7 months. For 12 month data: WT Control n = 71 dendrites (5 male mice), Tg2576: n = 85 dendrites (8 male mice), B6/J Control: n = 106 dendrites (7 mice, 4 males/3 females), 3xTg: n = 223 dendrites (14 mice, 6 males/8 females), APPNL-G-F: n = 130 dendrites (6 mice, 3 males/6 females). For 6–7 month data: B6/J Control: n = 47 dendrites (5 female mice), 3xTg: n = 77 dendrites (5 male mice), APPNL-G-F: n = 46 dendrites (7 mice, 4 males/3 females). All mice aged 12 months. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. WT wildtype, AUC area under Sholl curve