Fig. 1
RGC dendritic loss at 12 months in Tg2576, 3xTg-AD and APPNL-G-F mice. a Representative confocal images of RGCs from WT Control, Tg2576, B6/J Control, 3xTg-AD and APPNL-G-F. Arrows indicate axon. Scale bar 100 µm. b Corresponding dendritic field reconstructed in Imaris. c–f Sholl analysis of all reconstructed RGCs comparing single models to show significance. Line represents mean of group at each interval, shaded error zones represent ± SEM. Two-tailed Mann–Whitney U test at each interval distance, *p < 0.05, g number of dendrites at each branching level proximal to the cell body. Error bars correspond to SD. h Area under the Sholl curve. i Maximum number of Sholl intersections. j Total length of all dendrites. k Number of Hoechst-positive nuclei in the GCL. Column Scatterplots g bars represent mean from individual cells and error bars correspond to SD. Column Scatterplots h–j data points represent individual cells and error bars correspond to SD. Column Scatter plot k data points represent individual mice and error bars correspond to SD. WT Control: n = 74 cells (7 male mice), Tg2576: n = 58 cells (8 male mice), B6J Control: n = 114 cells (10 mice, 5 male/5 female), 3xTg-AD: n = 144 cells (12 mice, 6 male/6 female) and APPNL-G-F: n = 80 cells (6 mice, 3 male/3 female). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. All mice aged 12 months. WT wildtype, AUC area under the Sholl curve, GCL ganglion cell layer