Fig. 2

Supplementation with D-PUFAs decreases α-syn content and restores the levels of synaptic proteins. a Representative western blot probed with the anti-α-syn and anti-α-syn pSer129 antibodies. b, c Quantitative analysis of α-syn and α-syn pSer129 protein levels. d Immunoblotting detection for the CSP-α and SNAP25 synaptic proteins. e, f Histogram depicting CSP-α and SNAP25 immunoreactivity. Data were normalized to the loading control β-Actin. Results are expressed as mean ± SEM. n = 3. **p < 0.01 and *p < 0.05. Two-way ANOVA followed by Tukey’s post-hoc multiple comparisons test