Fig. 3

Calretinin immunohistochemistry in cortical plate of FASD and control brains at 24 WG. Random cortical distribution of CR interneurons in FASD brains with a predominance in deeper layers (asterisks) (a, b) compared to control cortical plate in which CR cells were more abundant in layer III (asterisk) (c). Quantitative analysis of cortical distribution of CR interneurons in each layer showing a predominance in layer III in normal brain (black arrow) and in deeper layers in FASD brains (red arrows) (d), associated with a lower global density in FASD brain compared to normal brain (e). Intrinsic distributions of CR-positive cells were compared in cortical layers I-VI from the control fetus and the FASD cases. Inserts within Fig. 3a, b ad c illustrate the bipolar morphology of CR interneurons at an original magnification of 400. Chi square analysis indicated that the cortical distributions significantly differed between the control case and both FASD cases (f). Scale bar = 0,18 mm