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Fig. 6 | Acta Neuropathologica Communications

Fig. 6

From: Impaired lipid metabolism in astrocytes underlies degeneration of cortical projection neurons in hereditary spastic paraplegia

Fig. 6

Alterations in LD size and distribution in ATL1 mutant astroglia. a, b LD-associated gene expression in WT, ATL1-P342S, H9, and ATL1-A161P #70 astroglia. c Visualization of LDs in WT, ATL1-P342S, H9, and ATL1-A161P #70 astroglial cells. d Distribution of LD size and e LD numbers per astroglial cell in WT and ATL1-P342S astroglia. f Distribution of LD size and g LDs numbers per astroglial cells in H9 and ATL1-A161P #70 astroglia. h Visualization of LDs in ATL1-P342S and ATL1-A161P #70 astroglia after DMSO or 1 µM GW3965 treatment for 3 days. i Distribution of LD size and j LD number per astroglial cell in ATL1-P342S astroglia after DMSO or 1 µM GW3965 treatment for 3 days. k Distribution of LD size and l LD number per astroglial cell in ATL1-A161P #70 astroglia after DMSO or 1 µM of GW3965 treatment for 3 days. m LD-associated gene expression in ATL1-P342S and ATL1-A161P #70 astroglia after DMSO or 1 µM of GW3965 treatment for 3 days. Red: LD540, green: GFAP, cyan: Hoechst. Scale bar: 20 µm. Data are represented as mean ± SEM. a, b, e, and g *p < 0.05 compared to WT or H9 by two-sided Student’s t-test. j, l, and m *p < 0.05 compared to ATL1-P342S and ATL1-A161P #70 astroglia after DMSO treatment, respectively. Chi square analysis was used to analyze the difference in the sizes of LDs

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