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Fig. 5 | Acta Neuropathologica Communications

Fig. 5

From: Impaired lipid metabolism in astrocytes underlies degeneration of cortical projection neurons in hereditary spastic paraplegia

Fig. 5

Cholesterol and astroglial cell-conditioned medium rescue axonal transport impairment and suppress axonal swellings in ATL1 mutant cortical PNs. a, b Representative kymographs of mitochondrial transport along axons of WT, H9, ATL1-P342S and ATL1-A161P #70 cortical PNs, with or without cholesterol (Cho) treatment as indicated. Scale bar: 5 µm. cf Percentage of anterograde moving mitochondria and motile mitochondria in WT, H9, ATL1-P342S, and ATL1-A161P #70 cortical PNs with exogenous Cho treatment. g, i Representative images of Tau staining indicating axonal swellings of 12-week ATL1-P342S and ATL1-A161P #70 cortical PNs after treatment with cholesterol or glial cell-conditioned medium (CM). WT glial CM for ATL1-P342S neurons and H9 glial CM for ATL1-A161P #70 neurons. Scale bar: 20 µm. h, j Quantification of axonal swellings per 100 µm of axon in ATL1-P342S and ATL1-A161P #70 cortical PNs with cholesterol or glial CM treatment. WT glial CM for ATL1-P342S neurons and H9 glial CM for ATL1-A161P #70 neurons. Data are represented as mean ± SEM. cf *p < 0.05 compared to WT and H9 cells, respectively. #p < 0.05 compared to ATL1-P342S or ATL1-A161P #70 by ANOVA. h, j)*p < 0.05 compared to ATL1-P342S control or ATL1-A161P #70 control by ANOVA

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