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Fig. 4 | Acta Neuropathologica Communications

Fig. 4

From: Impaired lipid metabolism in astrocytes underlies degeneration of cortical projection neurons in hereditary spastic paraplegia

Fig. 4

GW3965 treatment mitigates axonal defects in ATL1 mutant cortical PNs. a Synaptophysin transport in ATL1-P342S and ATL1-A161P #70 cortical PNs after treatment for 3 days with 5 µM GW3965 or DMSO. The x-axis indicates the axon length from proximal to distal areas. The y-axis indicates time-lapse duration in min (y = 5 min). Scale bar: 5 µm. b, c Anterograde (b) and retrograde (c) average moving velocities of SVs after GW3965 treatment for 3 days. d Ratio of moving SVs in relations to the total number of SVs after GW3965 treatment. e Ratio of anterograde moving SVs in relation to total SVs after GW3965 treatment. f Ratio of moving SVs per 100 µm of axon after GW3965 treatment. g Axonal swellings (Tau, red) of ATL1-P342S and ATL1-A161P #70 cortical PNs after GW3965 treatment for 7 days at D84. Scale bar: 20 µm. h Quantifications of axonal swellings of GW3965-treated ATL1-P342S and ATL1-A161P #70 cortical PNs. Data are presented as mean ± SEM from triplicate biological samples. *p < 0.05 compared to DMSO-treated ATL1-P342S or ATL1-A161P #70 cortical PNs using two-sided Student’s t-test

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