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Fig. 3 | Acta Neuropathologica Communications

Fig. 3

From: Impaired lipid metabolism in astrocytes underlies degeneration of cortical projection neurons in hereditary spastic paraplegia

Fig. 3

Defects of cholesterol metabolism in ATL1 mutant cortical PNs regular cell culture. a Representative Filipin III (blue) staining and corresponding phase images for WT, ATL1-P342S, ATL1-342-Cor, H9 and ATL1-A161P #70 cortical PNs in regular neural culture. The white circle highlights the cortical neurons in regular cell culture. Scale bar: 20 µm. be Filipin intensity fold-change in cell bodies (b) and axons (d) in WT, ATL1-P342S, and ATL1-342-Cor cortical PNs quantified by ImageJ. Filipin intensity fold-change in cell bodies (c) and (e) axons of H9 and ATL1-A161P #70 cortical PNs. f Cholesterol content in WT, ATL1-P342S, H9 and ATL1-A161P #70 regular neural cultures determined by a colorimetric method. g Relative ApoE content in culture medium of 10-week cortical PNs derived from WT and ATL1-P342S cells. There is a significant reduction in ApoE content in ATL1-P342S cortical PNs. h Significant reduction of ApoE content was also observed in ATL1-A161P #70 cortical PN cultures when comparing to WT cultures. Data are represented as mean ± SEM from 3 independent samples. *p < 0.05 for ATL1-P342S compared to WT and for ATL1-A161P #70 compared to H9. #p < 0.05 compared with ATL1-P342S

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