Skip to main content
Fig. 6 | Acta Neuropathologica Communications

Fig. 6

From: Infusion of blood from mice displaying cerebral amyloidosis accelerates amyloid pathology in animal models of Alzheimer’s disease

Fig. 6

Intra-venous administration of pure recombinant Aβ aggregates accelerates cerebral amyloid pathology. Young APP/PS1 double transgenic mice (30 days old) were injected i.v. with a preparation containing in vitro generated Aβ aggregates (200 µL), as described in the “Materials and Methods” section. Animals were injected once a week for 3 consecutive weeks and sacrificed at 180 days old. As controls, mice were injected i.v. with the same volume of PBS. To study the fate of the inoculum at short time periods, a group of animals injected with Aβ aggregates were sacrificed 50 days after the last injection. ac Representative pictures of 4G8 stained brain slices from experimental [RecHuAβ/80 days old (a)] or control groups [PBS/180 days old (b) and RecHuAβ/80 days old] mice. Scale bar at the left-bottom of the “RecHuAβ/80 days old” picture represents 100 µm and applies to all pictures. d The concentration of PBS insoluble Aβ42 in the brain of these animals was measured by ELISA. Symbols show the values of individual animals and the dotted line represents the threshold value used to calculate attack rate (in regards to PBS injected mice). The numbers on top indicate the ratio of animals over the threshold/total number of animals in the group. Data was analyzed by one-way ANOVA followed by the Tukey’s multiple comparison post hoc test. *P < 0.05; ***P < 0.001

Back to article page