Fig. 7

Effects of A2105T mutation on the cellular localization of DSCAML1 and the cell adherences. a Fluorescent microscopy images of L cells that stably express DSCAML1^WT or DSCAML1^A2105T. Scale bars: 10 µm. b Cell aggregation assay. L cells expressing DSCAML1^WT along with mCherry are mixed with L cells expressing EGFP, DSCAML1^WT with EGFP, or DSCAML1^A2105T with EGFP, respectively. Scale bars: 100 µm. c Quantitation of the number of 3 cell-clusters containing red and green cells in the field for each cell line (n = 4, ***p < 0.001, Student’s t-test, Error bars: s.e.m.)