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Table 1 List of antibodies and reagents for imaging

From: Retinal capillary degeneration and blood-retinal barrier disruption in murine models of Alzheimer’s disease

Antibodies or reagents

Source species

Dilution

Application

Commercial source

Catalog. #

Primary antibody

PDGFRβ pAb

Goat

1:200

IF

R&D systems

AF385

4G8 mAb

Mouse

1:200

IF

Biolegend

800701

Alexa Fluor 488-conjugated tomato lectin

Lycopersicon esculentum

1:200

IF

Dylight

DL-1174

40 (11A50-B10) mAb

Mouse

1:200

IF

Biolegend

805401

CD31 pAb

Rabbit

1:100

IF

Abcam

ab28364

Zonula Occluden-1 pAb

Rabbit

1:50

WB

Thermofisher

61-7300

Claudin-1 pAb

Rabbit

1:75

WB

Thermofisher

51-9000

phospho-NF-κB p65 Ser536 mAb

Rabbit

1:1000

WB

Cell Signaling Technology

3033S

NF-κB p65 mAb

Rabbit

1:1000

WB

Cell Signaling Technology

8242S

β-actin

Mouse

1:1000

WB

Santa-Cruz Biotechnology

Sc-47778

Secondary antibody

Cy3 (anti-rabbit, anti-goat)

Donkey

1:200

IF

Jackson ImmunoResearch Laboratories

Cy5 (anti-mouse, anti-rabbit)

Donkey

1:200

IF

Jackson ImmunoResearch Laboratories

Peroxidase Goat Anti-Rabbit IgG (H + L)

Goat

1:10000

WB

Jackson ImmunoResearch Laboratories

Peroxidase Goat Anti-Mouse IgG (H + L)

Goat

1:10000

WB

Jackson ImmunoResearch Laboratories

In vivo retinal live imaging

Fluorescein (322.31 Da)

N/A

2%

IVRLI

Dailymed

17478-250-20

Ex vivo retinal imaging

FITC-dextran (2000kD)

N/A

0.25%

EVRI

Sigma Aldrich

FD2000s-5G

Texas Red-dextran (3kD)

N/A

0.25%

EVRI

Thermofisher

D1829

Thioflavin-S

N/A

1%

IF

Sigma-Aldrich

T1892

Degenerated capillary quantification (PAS)

Periodic Acid

N/A

35 mM

DCQ

Milliporesigma

P7875

Schiff

N/A

100%

DCQ

Sigma Aldrich

3952016

  1. IF Immunofluorescence, WB western blot, pAb polyclonal antibody, mAb monoclonal antibody, IVRLI in vivo retinal live imaging, EVRI ex vivo retinal imaging, DCQ degenerated capillary quantification, N/A not applicable, if not marked otherwise, antibody dilution is indicated for immunofluorescent assay