Antibodies or reagents | Source species | Dilution | Application | Commercial source | Catalog. # |
---|---|---|---|---|---|
Primary antibody | |||||
PDGFRβ pAb | Goat | 1:200 | IF | R&D systems | AF385 |
4G8 mAb | Mouse | 1:200 | IF | Biolegend | 800701 |
Alexa Fluor 488-conjugated tomato lectin | Lycopersicon esculentum | 1:200 | IF | Dylight | DL-1174 |
Aβ40 (11A50-B10) mAb | Mouse | 1:200 | IF | Biolegend | 805401 |
CD31 pAb | Rabbit | 1:100 | IF | Abcam | ab28364 |
Zonula Occluden-1 pAb | Rabbit | 1:50 | WB | Thermofisher | 61-7300 |
Claudin-1 pAb | Rabbit | 1:75 | WB | Thermofisher | 51-9000 |
phospho-NF-κB p65 Ser536 mAb | Rabbit | 1:1000 | WB | Cell Signaling Technology | 3033S |
NF-κB p65 mAb | Rabbit | 1:1000 | WB | Cell Signaling Technology | 8242S |
β-actin | Mouse | 1:1000 | WB | Santa-Cruz Biotechnology | Sc-47778 |
Secondary antibody | |||||
Cy3 (anti-rabbit, anti-goat) | Donkey | 1:200 | IF | Jackson ImmunoResearch Laboratories | |
Cy5 (anti-mouse, anti-rabbit) | Donkey | 1:200 | IF | Jackson ImmunoResearch Laboratories | |
Peroxidase Goat Anti-Rabbit IgG (H + L) | Goat | 1:10000 | WB | Jackson ImmunoResearch Laboratories | |
Peroxidase Goat Anti-Mouse IgG (H + L) | Goat | 1:10000 | WB | Jackson ImmunoResearch Laboratories | |
In vivo retinal live imaging | |||||
Fluorescein (322.31 Da) | N/A | 2% | IVRLI | Dailymed | 17478-250-20 |
Ex vivo retinal imaging | |||||
FITC-dextran (2000kD) | N/A | 0.25% | EVRI | Sigma Aldrich | FD2000s-5G |
Texas Red-dextran (3kD) | N/A | 0.25% | EVRI | Thermofisher | D1829 |
Thioflavin-S | N/A | 1% | IF | Sigma-Aldrich | T1892 |
Degenerated capillary quantification (PAS) | |||||
Periodic Acid | N/A | 35 mM | DCQ | Milliporesigma | P7875 |
Schiff | N/A | 100% | DCQ | Sigma Aldrich | 3952016 |