Fig. 4

iTTBK1 Tg mice exhibit increased neuroinflammation. a–f Representative hippocampal brain sections from control and iTTBK1 Tg mice immunostained for GFAP (a, b) and Iba1 (d, e). Scale bar = 100 μm. c, f Induction of the transgene in iTTBK1 Tg mice significantly increased GFAP and Iba1 immunoreactivity in the hippocampus. ***p < 0.0001 by two-tailed t test. DML dentate molecular layer, SLM stratum laculosum moleculare. g–j Representative immunostaining of GFAP and Iba1 in the cerebellum was increased in all iTTBK1 Tg animals compared to control animals. g, h GFAP, i, j Iba1 Scale Bar = 200 μm. Immunostaining of GFAP and Iba1 in the cerebellum was increased in all iTTBK1 Tg animals compared to control animals but the signal was too diffuse to quantify