Fig. 3

iTTBK1 Tg mice exhibit hyperactivity and impairments in spatial memory. a Open field testing of control and iTTBK1 Tg mice. iTTBK1 Tg are hyperactive compared to controls, ***p < 0.001. b Open field testing of control and iTTBK1 Tg mice. iTTBK1 Tg travel less in the center of the arena compared to controls. Percentage of distance travelled in the center of the field versus the periphery, ***p < 0.001. c iTTBK1 Tg mice have impaired learning of the location of the escape hole during Barnes maze training trials (mean ± SEM), ***p = 0.001. In addition to the main effect of genotype (p = 0.046), there was also a significant main effect of sex (p = 0.001). d Female control mice learn the location of the escape hole during Barnes maze training, while iTTBK1 Tg female mice do not, **p = 0.02. e Both male control and iTTBK1 Tg mice fail to learn the location of the escape hole during Barnes maze training (trial #4: p = 0.52). f On the day of the Barnes maze probe trial, iTTBK1 Tg mice do not exhibit significant impairments in locating the escape hole (main effect of genotype, p = 0.16). However, as in the training trials, there were significant sex effects (main effect of sex, p = 0.003; genotype × sex interaction, p = 0.04). g On the day of the Barnes maze probe trial, female control mice are able to find the escape hole significantly faster than female iTTBK1 Tg mice, ***p = 0.007. h On the day of the probe trial, male control and iTTBK1 Tg mice exhibit similar times to find the escape hole