Fig. 1

Evaluation of retinal thickness in three to 25 week old unaffected and MPS IIIA mice. Representative images of haematoxylin and eosin-stained retina from 25 week old unaffected and MPS IIIA mice are shown. Photos show central (a, b) and peripheral (c, d) retina. e Total retinal thickness, defined as the distance from the ganglion cell layer (GCL) to the photoreceptor segments (PS), was quantified in mice aged 3–25 weeks. The thickness of the inner plexiform layer (IPL; f), inner nuclear layer (INL; g) and outer plexiform layer (OPL; h) was determined. The thickness of (i) and number of rows of cells in (j) the outer nuclear layer (ONL) is shown. Representative images of TUNEL labelling in 25 week old unaffected (k) and MPS IIIA (l) mouse central retina. m Quantification of TUNEL-positive cells in the retina of unaffected and MPS IIIA mice aged three to 25 weeks. Scale bar: a–d = 50 µm; k, l = 25 µm. Data are expressed as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001