Fig. 4
From: Anti-Aβ antibodies bound to neuritic plaques enhance microglia activity and mitigate tau pathology
Human tau overexpression affects the clustering of microglia around Aβ plaques. a Percentage of the area of the ipsi- and contralateral hippocampus covered by Iba1 positive staining. b Representative images of the distribution of Iba1 staining (green) around Aβ plaques (blue) in 5xFAD/AAV-GFP and 5xFAD/AAV-tau mice. To quantify the distribution of microglia in plaque periphery, the region of interest (ROI, delineated by the red line) was defined by including a 5-µm wide rim around the detected Aβ plaque area (periphery shown in blue). The percentage coverage by microglial markers (Iba1 green staining delineated by the white line) was measured within the ROI. c Percentage of the ROI area covered by Iba1 staining in both the ipsi- and contralateral hippocampus. Note the significant decrease in the 5xFAD/AAV-tau mice. d Percentage of the same ROI area covered by CD68 staining. Statistical analysis: two-way ANOVA with post hoc Sidak’s multiple comparison test; ns non-significant, *p < 0.05, ***p < 0.001; 5xFAD/AAV-GFP: n = 15 mice, 5xFAD/AAV-tau: n = 15 mice