Fig. 5
From: In vivo RyR1 reduction in muscle triggers a core-like myopathy
A generalized structural disorganization is observed in EDL muscle fibers. a EDL fibers from D75 CTRL and RyR1-Rec mice were stained with antibodies against RyR1 (green), triadin (red) and alpha-actinin (white). Bar 10 µm and 2 µm in inset. b Electron microscopy analysis of longitudinal EDL section of D75 RyR1-Rec mice. The upper left fiber has a normal structure, the adjacent lower fiber is extremely disorganized, with large stacks of membranes (up to 15 stacks, arrows) extending on few µm long. The inset presents two multiple triads, on the left one the membranes corresponding to T-tubules have been colored with light yellow and the SR sheets with light blue to allow a better visualization. Some electron dense material can be seen within the SR segment, all along the contact site with the adjacent T-tubule, which could correspond to accumulation of proteins. Bars 1 µm