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Fig. 2 | Acta Neuropathologica Communications

Fig. 2

From: A novel structure associated with aging is augmented in the DPP6-KO mouse brain

Fig. 2

Controls for immunofluorescence labeling. A IF labeling of NeuN+ puncta with Alexa fluor-488 goat anti-Rabbit IgG secondary antibody (1:800, ThermoFisher). The NeuN labeled puncta only can be observed at 488, and not at 555, showing that the NeuN labeling is not caused by autofluorescence. B Double labeling showing the colocalization of NeuN mouse antibody (green) and rabbit antibody (red). Scale bar = 50 μm. C IF labeling only with Alexa fluor-488 goat anti-Rabbit IgG secondary antibody (1:800, ThermoFisher) control; without the primary NeuN antibody, the puncta cannot be observed. Scale bar = 50 μm. sp, stratum pyramidale; sr, stratum radiatum

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