Fig. 3

Bexarotene reverses white matter damaged induced by cisplatin treatment. a Representative overview images of myelin staining using Black Gold II following bexarotene treatment in cisplatin-treated animals (n = 4). Black squares indicate the two areas analyzed—cingulate and sensorimotor cortices. Scale bar = 1000 μM. b, c Quantification of percent positive area to indicate myelin density in the cingulate (b) and sensorimotor (c) cortex. For the cingulate cortex, images were skeletonized and the coherency index was quantified to assess the complexity of fibers (Scale bar = 100 μM). Results are expressed as mean ± SD. Two-way ANOVA followed by Tukey’s multiple comparisons test. *p < 0.05, ***p < 0.001, ****p < 0.0001