Intracellular dynamics of Ataxin-2 in the human brains with normal and frontotemporal lobar degeneration with TDP-43 inclusions

Table 3 Colocalization coefficient values from all evaluated brain regions of the control cases

Brain regions	Coefficients	Molecules immunolabeled with ATXN2
Brain regions	Coefficients	Calnexin	RPS6	LAMP1	GLG1	PABP1	TDP-43
Temporal cortex	PCC	0.4024	0.7520^#	0.0683	0.0717	0.7388^#	− 0.3033
	(SD)	0.0761	0.1161	0.0719	0.0544	0.0867	0.0232
	tM	0.7261*	0.9399**	0.4245	0.5203	0.897**	0.1353
	(SD)	0.0788	0.0486	0.1004	0.0501	0.0969	0.0601
Hippocampal CA1	PCC	0.4050	0.6021^#	− 0.0423	− 0.1191	0.7289^#	− 0.0024
	(SD)	0.1060	0.1817	0.1583	0.1720	0.0969	0.2114
	tM	0.7310*	0.8833**	0.4607	0.4284	0.9324**	0.4782
	(SD)	0.0340	0.0719	0.0277	0.1983	0.0239	0.0303
Subiculum	PCC	0.4559	0.6872^#	− 0.0366	− 0.2399	0.7087^#	− 0.1173
	(SD)	0.1315	0.0998	0.0265	0.1177	0.1319	0.2762
	tM	0.8064**	0.9257**	0.5100	0.3347	0.9237**	0.4434
	(SD)	0.0846	0.0178	0.0842	0.0958	0.0392	0.0625
Parahippocampal gyrus	PCC	0.4421	0.6875^#	0.1103	− 0.1156	0.7730^#	− 0.1112
	(SD)	0.0727	0.0360	0.1564	0.1005	0.1309	0.1103
	tM	0.7512*	0.9218**	0.6145	0.4643	0.9627**	0.4236
	(SD)	0.1079	0.0243	0.0624	0.0704	0.0219	0.0355

Pearson’s correlation coefficients above threshold (PCC) and Manders’ colocalization coefficients above threshold (tM) of control cases were calculated according to each cytoplasmic ROI. Each mean value from at least fifteen ROIs was plotted with standard deviation. PCC represents the overall linear relationship from − 1 (perfectly inversely related) to 1 (perfectly related). Any value greater than − 0.5 and less than 0.5 is taken to mean that the two probes are unrelated. PCC values showing a significant relation are indicated in italics letters with ^# in the chart. tM shows the fraction of ATXN2’s fluorescence in compartments containing fluorescence of its counterpart molecule and ranges from 0 to 1. We classified a tM as high (0.8 ≤ tM, indicated in italics letters with ** in the chart), moderate (0.65 ≤ tM < 0.8, indicated in italics letters with *), or low (tM < 0.65, no highlights)

ISSN: 2051-5960