|
Brain regions
|
Coefficients
|
Molecules immunolabeled with ATXN2
|
|---|
|
Calnexin
|
RPS6
|
LAMP1
|
GLG1
|
PABP1
|
TDP-43
|
|---|
|
Temporal cortex
|
PCC
|
0.4024
|
0.7520#
|
0.0683
|
0.0717
|
0.7388#
|
− 0.3033
|
| |
(SD)
|
0.0761
|
0.1161
|
0.0719
|
0.0544
|
0.0867
|
0.0232
|
| |
tM
|
0.7261*
|
0.9399**
|
0.4245
|
0.5203
|
0.897**
|
0.1353
|
| |
(SD)
|
0.0788
|
0.0486
|
0.1004
|
0.0501
|
0.0969
|
0.0601
|
|
Hippocampal CA1
|
PCC
|
0.4050
|
0.6021#
|
− 0.0423
|
− 0.1191
|
0.7289#
|
− 0.0024
|
| |
(SD)
|
0.1060
|
0.1817
|
0.1583
|
0.1720
|
0.0969
|
0.2114
|
| |
tM
|
0.7310*
|
0.8833**
|
0.4607
|
0.4284
|
0.9324**
|
0.4782
|
| |
(SD)
|
0.0340
|
0.0719
|
0.0277
|
0.1983
|
0.0239
|
0.0303
|
|
Subiculum
|
PCC
|
0.4559
|
0.6872#
|
− 0.0366
|
− 0.2399
|
0.7087#
|
− 0.1173
|
| |
(SD)
|
0.1315
|
0.0998
|
0.0265
|
0.1177
|
0.1319
|
0.2762
|
| |
tM
|
0.8064**
|
0.9257**
|
0.5100
|
0.3347
|
0.9237**
|
0.4434
|
| |
(SD)
|
0.0846
|
0.0178
|
0.0842
|
0.0958
|
0.0392
|
0.0625
|
|
Parahippocampal gyrus
|
PCC
|
0.4421
|
0.6875#
|
0.1103
|
− 0.1156
|
0.7730#
|
− 0.1112
|
| |
(SD)
|
0.0727
|
0.0360
|
0.1564
|
0.1005
|
0.1309
|
0.1103
|
| |
tM
|
0.7512*
|
0.9218**
|
0.6145
|
0.4643
|
0.9627**
|
0.4236
|
| |
(SD)
|
0.1079
|
0.0243
|
0.0624
|
0.0704
|
0.0219
|
0.0355
|
- Pearson’s correlation coefficients above threshold (PCC) and Manders’ colocalization coefficients above threshold (tM) of control cases were calculated according to each cytoplasmic ROI. Each mean value from at least fifteen ROIs was plotted with standard deviation. PCC represents the overall linear relationship from − 1 (perfectly inversely related) to 1 (perfectly related). Any value greater than − 0.5 and less than 0.5 is taken to mean that the two probes are unrelated. PCC values showing a significant relation are indicated in italics letters with # in the chart. tM shows the fraction of ATXN2’s fluorescence in compartments containing fluorescence of its counterpart molecule and ranges from 0 to 1. We classified a tM as high (0.8 ≤ tM, indicated in italics letters with ** in the chart), moderate (0.65 ≤ tM < 0.8, indicated in italics letters with *), or low (tM < 0.65, no highlights)
