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Table 3 Colocalization coefficient values from all evaluated brain regions of the control cases

From: Intracellular dynamics of Ataxin-2 in the human brains with normal and frontotemporal lobar degeneration with TDP-43 inclusions

Brain regions Coefficients Molecules immunolabeled with ATXN2
Calnexin RPS6 LAMP1 GLG1 PABP1 TDP-43
Temporal cortex PCC 0.4024 0.7520# 0.0683 0.0717 0.7388# − 0.3033
  (SD) 0.0761 0.1161 0.0719 0.0544 0.0867 0.0232
  tM 0.7261* 0.9399** 0.4245 0.5203 0.897** 0.1353
  (SD) 0.0788 0.0486 0.1004 0.0501 0.0969 0.0601
Hippocampal CA1 PCC 0.4050 0.6021# − 0.0423 − 0.1191 0.7289# − 0.0024
  (SD) 0.1060 0.1817 0.1583 0.1720 0.0969 0.2114
  tM 0.7310* 0.8833** 0.4607 0.4284 0.9324** 0.4782
  (SD) 0.0340 0.0719 0.0277 0.1983 0.0239 0.0303
Subiculum PCC 0.4559 0.6872# − 0.0366 − 0.2399 0.7087# − 0.1173
  (SD) 0.1315 0.0998 0.0265 0.1177 0.1319 0.2762
  tM 0.8064** 0.9257** 0.5100 0.3347 0.9237** 0.4434
  (SD) 0.0846 0.0178 0.0842 0.0958 0.0392 0.0625
Parahippocampal gyrus PCC 0.4421 0.6875# 0.1103 − 0.1156 0.7730# − 0.1112
  (SD) 0.0727 0.0360 0.1564 0.1005 0.1309 0.1103
  tM 0.7512* 0.9218** 0.6145 0.4643 0.9627** 0.4236
  (SD) 0.1079 0.0243 0.0624 0.0704 0.0219 0.0355
  1. Pearson’s correlation coefficients above threshold (PCC) and Manders’ colocalization coefficients above threshold (tM) of control cases were calculated according to each cytoplasmic ROI. Each mean value from at least fifteen ROIs was plotted with standard deviation. PCC represents the overall linear relationship from − 1 (perfectly inversely related) to 1 (perfectly related). Any value greater than − 0.5 and less than 0.5 is taken to mean that the two probes are unrelated. PCC values showing a significant relation are indicated in italics letters with # in the chart. tM shows the fraction of ATXN2’s fluorescence in compartments containing fluorescence of its counterpart molecule and ranges from 0 to 1. We classified a tM as high (0.8 ≤ tM, indicated in italics letters with ** in the chart), moderate (0.65 ≤ tM < 0.8, indicated in italics letters with *), or low (tM < 0.65, no highlights)