Table 2 Primary antibodies used in this study
Primary antibodies | Type | Source | Dilution |
|---|---|---|---|
Human Ataxin-2, 713-904 | Mouse, monoclonal | BD biosciences (611378) | 1:1000 (IPL), 1:500 (IF), 1:250–1000 (WB) |
Human Ataxin-2, 1293-1313 | Rabbit, polyclonal | Thermofisher (#PA5-78845) | 1:2000 (IF), 1:2000 (WB) |
Human ribosomal protein S6 (5G10) | Rabbit, monoclonal | CST (#2217) | 1:500 (IF), 1:3000 (WB) |
Human PolyA-binding protein 1 | Rabbit, polyclonal | CST (#4992) | 1:500 (IF) |
Human Calnexin (C5C9) | Rabbit, monoclonal | CST (#2679) | 1:500 (IF) |
Human Lysosome associated membrane protein 1 | Rabbit, polyclonal | Abcam (ab24170) | 1:1000 (IF) |
Human Golgi Glycoprotein 1 | Rabbit, polyclonal | Abcam (ab103439) | 1:500 (IF) |
Human TDP-43 (2E2-D3) | Mouse, monoclonal | Abnova (H00023435-M01) | 1:500 (IF) |
Phosphorylated TDP-43 (pS409/410) | Rabbit, polyclonal | Made by Dr. M. Hasegawa [16] | 1:1000 (WB) |
Anti-Glyceraldehyde-3-Phosphate Dehydrogenase Antibody (6C5) | Mouse, monoclonal | Chemicon (MAB374) | 1:3000 (WB) |