Fig. 7
Semi-quantitative immunohistochemical analysis of human neuronal ATXN2. The brain sections of temporal neocortex from three control cases and three FTLD-TDP cases were immunostained with the antibodies for ATXN2, RPS6, and PABP1 by the same procedure. The signal intensities within each neuronal cytoplasm from the fluorescent images were semi-quantified and statistically compared. a Representative immunofluorescent images and automatically determined ROIs within each neuronal cytoplasm. b Integrated density and corrected total cell fluorescence (CTCF) values per unit area (μm2) for ATXN2, RPS6, and PABP1 were calculated from fifty ROIs and plotted. Data are mean ± standard deviation, and statistical comparisons were made by unpaired Student T tests. The signal intensity of ATXN2 in the FTLD-TDP cases was significantly reduced compared with controls (*P ≤ 0.05), but its related molecules RPS6 and PABP1, showed no such significant reduction