Fig. 6

Polysome profiling of ATXN2 of mouse brain homogenates. Mouse whole brain homogenates were fractionated in a 10–50% (w/v) sucrose density-gradient, collected with a monitoring RNA absorption curve (absorbance at 260 nm: A260), and analyzed by western blotting. a Analysis of whole brain extract from a mouse showed the sedimentation of ATXN2 and ribosomal subunit RPS6 in both monosomal and polysomal fractions. b EDTA treatment disrupted the sedimentation of ATXN2 and RPS6 with polysomal fractions. The cropped blots are presented for clarity and conciseness