Fig. 5

Colocalization image of ATXN2 and pTDP-43 in the dentate gyrus of FTLD-TDP brain. The parahippocampal gyrus and dentate gyrus of FTLD-TDP cases were immunostained by double-immunofluorescent labeling using the mouse monoclonal anti-ATXN2 antibody and the rabbit polyclonal anti-pTDP-43 antibody or immunoperoxidase labeling using the mouse monoclonal anti-ATXN2 antibody. Each representative image is presented. a The double-immunofluorescent images of parahippocampal gyrus showed that part of pTDP-43 positive DNs (red image, arrowheads) were positive to ATXN2 (green image, arrows), and that both signals colocalized in the merged image (asterisks). Representative DN is also presented in the magnified images. b The immunoperoxidase labeling of parahippocampal gyrus showed a weak ATXN2-immunoreactivity resembling TDP-43 positive DNs (arrows). c The double-immunofluorescent images of the dentate gyrus showed the colocalization of pTDP-43 and ATXN2 in most of NCIs formed in the granule cells. d The immunoperoxidase labeling of the dentate gyrus showed a weak ATXN2-immunoreactivity resembling TDP-43 positive NCIs (arrows). In a and c, DAPI staining is included in the merged image, and pseudo coloring was used for each channel. The scale bars included in each image represent 30 µm