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Fig. 1 | Acta Neuropathologica Communications

Fig. 1

From: Intracellular dynamics of Ataxin-2 in the human brains with normal and frontotemporal lobar degeneration with TDP-43 inclusions

Fig. 1

Verification of ATXN2 antibodies. Two different ATXN2 antibodies were used in this study. Their specificities were confirmed by western blotting of cultured cells transfected with ATXN2 expression plasmids, pFN21A-Halo-ATXN2 (lane 1, 3) and pcDNA3-Flag-ATXN2 (lane 2, 4). The left blot (lane 1, 2) and the right one (lane 3, 4) were incubated with mouse monoclonal antibody (BD Biosciences, aa713-904) and rabbit polyclonal antibody (Thermofisher, aa1293-1313), respectively. Halo- and Flag-tagged ATXN2 were electrophoresed at approximately 220 kDa and 160 kDa, respectively

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