Fig. 8

Cttnbp2 M120I mutant mice exhibit reduced social interaction and neuronal activation. a Flowchart of behavioral assays used in this report. Two hours after the last assay, mouse brains were fixed and subjected to C-FOS staining. b Open field. c Elevated plus maze. d Reduced reciprocal social interaction (RSI) in M120I mutant mice. e Reduced sociability of M120I mutant mice in three-chamber test. f Reduced novelty preference of M120I mutant mice in three-chamber test. Heat maps of movement paths of mice, actual interaction time, the difference of interaction time between the left and right chambers, and preference index are shown. g C-FOS staining of M120I mutant mice after social stimulation. DG dentate gyrus, RSP retrosplenial cortex, MO motor cortex, SOP somatosensory cortex, BLA basal lateral amygdala. Data represent mean ± SEM and the results of individual mice are shown. d Two-tailed Mann–Whitney test. e–f A paired t test was used to compare total interaction times for the object (Ob) and stranger 1 (S1) or for S1 and stranger 2 (S2). To compare interaction times or preference indexes, an unpaired t-test or Mann–Whitney test was used. For (g), an unpaired t-test or Mann–Whitney test was used. All statistical data and exact sample sizes are available in Additional file 2: Table S1. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ns, not significant