Fig. 7

M120I and D570Y mutations of CTTNBP2 reduce dendritic spine density in vivo. a, c Design and mutation sites of Cttnbp2 M120I and D570Y knockin mice using CRISPR/Cas9 editing technology as indicated. Sequencing results of heterozygous mice are shown. b, d Cresyl violet staining reveals no obvious brain anatomical defect of Cttnbp2 M120I and D570Y knockin heterozygous mice. e–j Both M120I and D570Y mutations reduce dendritic spine density and only the M120I mutant alters dendritic spine morphology. Dendritic spine analysis of M120I (e–g) or D570Y (h–j) mutant mice based on the signals of Thy1-YFP. e, h Representative images of the first dendritic branches of CA1 neurons and quantification results are shown. f, i Quantification of dendritic spine density and morphology. Data represent mean ± SEM and the results of individual neurons are also shown. For (f), N = 4 mice and n = 40 neurons. For (i), N = 3 mice and n = 30 neurons. g, j Cumulative probability of individual spine morphology in M120I (MI) or D570Y (DY) mice as indicated. For M120I spine density and D570Y spine width and length, two-tailed Mann–Whitney tests were performed. For M120I spine width and length and D570Y spine density, two-tailed unpaired t tests were performed. For cumulative probabilities, Kolmogorov–Smirnov (K–S) tests were performed. All statistical data and exact sample sizes are available in Additional file 2: Table S1. *P < 0.05; **P < 0.01; ***P < 0.001; ns, not significant. Scale bar: (b, d) 1 mm; (e, h) 2.5 μm