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Fig. 5 | Acta Neuropathologica Communications

Fig. 5

From: Mild hypoxia triggers transient blood–brain barrier disruption: a fundamental protective role for microglia

Fig. 5

Microglial depletion results in greater loss of endothelial tight junction protein expression during CMH. a, c Frozen brain sections (images captured in brainstem) taken from mice fed normal chow or PLX5622-containing chow and maintained under hypoxic conditions for 7 days were stained for CD31 (AlexaFluor-488) and ZO-1 (Cy-3) in a or CD31 (AlexaFluor-488) and occludin (Cy-3) in c. Scale bar = 50 μm. b, d. Quantification of endothelial expression of ZO-1 (b) or occludin (d) on blood vessels in the brainstem. Results are expressed as the mean ± SEM (n = 4 mice/group). *p < 0.05. Note that under hypoxic conditions, brains of PLX5622-fed showed focal areas in which blood vessels showed diminished expression of ZO-1 and occludin (see arrows in a, c)

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