Fig. 7From: rAAV-related therapy fully rescues myonuclear and myofilament function in X-linked myotubular myopathyCentral nucleation in the absence of disease does not affect contractile force production. Tibialis anterior muscles from wild type mice were treated with notexin to induce degeneration, followed by a period of 8 weeks for full muscle regeneration. Uninjected contralateral muscles served as controls. Muscles injured and regenerated for 8 weeks displayed similar histological parameters to the uninjured controls: proportions of type I and type II fibres (a–d, g), and cross-sectional areas of fibres (e, f, h). However, regenerated muscles retained a large fraction of central nuclei, absent in controls (e, f, i). Despite this change, contractile force in skinned fibres was similar in both groups (j). WGA (wheat-germ agglutinin), CSA, cross-sectional area. Scale bars: a–d, 50 μm; e, f, 25 μm. Individual data points in g–i correspond to individual animals, and in j to individual fibres pooled from 4 animals. Graphs show mean ± S.D. *denotes a significant difference compared to the healthy group (P < 0.05, t-test)Back to article page