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Fig. 7 | Acta Neuropathologica Communications

Fig. 7

From: rAAV-related therapy fully rescues myonuclear and myofilament function in X-linked myotubular myopathy

Fig. 7

Central nucleation in the absence of disease does not affect contractile force production. Tibialis anterior muscles from wild type mice were treated with notexin to induce degeneration, followed by a period of 8 weeks for full muscle regeneration. Uninjected contralateral muscles served as controls. Muscles injured and regenerated for 8 weeks displayed similar histological parameters to the uninjured controls: proportions of type I and type II fibres (a–d, g), and cross-sectional areas of fibres (e, f, h). However, regenerated muscles retained a large fraction of central nuclei, absent in controls (e, f, i). Despite this change, contractile force in skinned fibres was similar in both groups (j). WGA (wheat-germ agglutinin), CSA, cross-sectional area. Scale bars: ad, 50 μm; e, f, 25 μm. Individual data points in g–i correspond to individual animals, and in j to individual fibres pooled from 4 animals. Graphs show mean ± S.D. *denotes a significant difference compared to the healthy group (P < 0.05, t-test)

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