Fig. 6

Iba1^low population co-expresses of L-Ferritin, HLA-DR, and CD74. To determine the co-expression of dysfunctional marker, L-Ferritin, with other immunophenotype MOIs on the Iba1^low population, Iba1 was immunofluorescently co-labelled with L-Ferritin and one other MOI; HLA-DR (a–d), CD74 (e–h), CD206 (i–l), CD32 (m–p), and TMEM119 (q–t). All Iba1-L-Ferritin-MOI cells were pooled from all normal and AD cases and plotted for their Iba1 average intensity against L-Ferritin average intensity to identify the Iba1^low population (a, e, i, m, q). All Iba1^low cells from all normal and AD cases were subsequently plotted for their MOI average intensity against L-Ferritin average intensity to identify three L-Ferritin-MOI populations; 1. L-Ferritin^high MOI^low, 2. L-Ferritin^high MOI^high, and 3. L-Ferritin^low MOI^high (b, f, j, n, r). An example of the L-Ferritin-MOI gating is shown on a single AD case for each MOI (c, g, k, o, s). The abundance of each L-Ferritin-MOI population was quantified as a percentage of total Iba1^low cells identified with each triple label (d, h, l, p, t); data presented as mean (n = 8)