Fig. 4

Iba1^low MOI^high population shows higher expression of activation markers than the respective Iba1^high MOI^high populations. Iba1-MOI populations were identified by immunofluorescent co-labelling of Iba1 with markers CD45 (a), HLA-DR (b), CD14 (c), CD74 (d), CD33 (e), CD206 (f), CD32 (g), CD163 (h), P2RY12 (I), and TMEM119 (J). The mean population intensity of the markers was measured for the Iba1^low MOI^high and Iba1^high MOI^high populations in each normal and AD case. The mean population intensities of each MOI were compared between the Iba1-MOI populations, and normal and AD using a two-way ANOVA with Tukey’s multiple comparisons test. Data are presented as mean ± SD (n = 7-8). Significance of differences between Iba1-MOI populations, and normal and AD: ****p ≤ 0.0001, ***p ≤ 0.001, **p ≤ 0.01, *p ≤ 0.05