Fig. 3

Iba1^low MOI^high populations are increased in AD. Iba1-MOI populations were identified by immunofluorescent co-labelling of Iba1 with markers CD45 (a), HLA-DR (b), CD14 (c), CD74 (d), CD33 (e), CD206 (f), CD32 (g), CD163 (h), P2RY12 (I), TMEM119 (j), and L-Ferritin (k). Following the single cell Iba1-MOI analysis, the abundance of the Iba1^low MOI^high, Iba1^high MOI^high, and Iba1^high MOI^low populations were quantified as a percentage of the total Iba1-MOI population identified in each normal and AD case. The abundance of each Iba1-MOI population was compared between control and AD using a Mann–Whitney test. Data are presented as mean abundance ± SD (n = 7 or 8). Significance of differences between normal and AD: ***p ≤ 0.001, **p ≤ 0.01, *p ≤ 0.05