Fig. 2

Novel single cell analysis identifies change in Iba1-MOI cell populations in AD. Iba1 was immunofluorescently co-labelled with markers in the normal and AD human middle temporal gyrus. The single cell Iba1-MOI analysis was used to quantify changes in the proportions of three Iba1-MOI populations in AD: 1. Iba1^low MOI^high, 2. Iba1^high MOI^high, and 3. Iba1^high MOI^low (a). The MOIs immunohistochemically stained for and quantified included CD45 (b, c), HLA-DR (d, e), CD14 (f, g), CD74 (h, i), CD33 (j, k), CD206 (l), CD32 (m, n), CD163 (o, p), P2RY12 (q, r), TMEM119 (s, t), and L-Ferritin (u, v). Example contour plots of Iba1 against MOI average intensity with the gates of the Iba1-MOI populations are presented for one normal and one AD case for CD45 (b), HLA-DR (d), CD14 (f), CD74 (h), CD33 (j), CD206 (l), CD32 (n), CD163 (p), P2RY12 (q), TMEM119 (s), and L-Ferritin (u). The net change in the distribution of the Iba1-MOI average intensities in AD is presented as a heat map of the normalised change (c, e, g, i, k, m, o, r, t, v). The x- and y-axis labels and heatmap scale are presented in (w)