Fig. 4

Inflammation markers and microglia shape descriptors 6 weeks after TBI in WT and MASP-2^−/− mice. a, b MASP-2 deletion did not affect the level of inflammatory markers such as glial fibrillary acidic protein (GFAP) and CD11b. The data is shown as a scatter dot plot, line at mean ± SEM (n = 5–6); unpaired t test. c Shape descriptors of CD11b positive cells showed that at 6 weeks after TBI microglia are still activated, having increased area, perimeter, Feret diameter and ramifications (indicated by lower circularity) higher in ipsi than in contralateral side. The drawings beside the y-axis indicate the expected values for each parameter depending on cell shape or symmetry. Microglia morphology did not differ between the two genotypes. The data is shown as a scatter dot plot, line at mean ± SEM (n = 6); Two-way Anova followed by Sidak’s post hoc test. d Representative high-magnification images of CD11b positive cells showing activated microglia in the ipsilateral side of both WT and MASP-2^−/− TBI mice, scale bar 20 µm