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Fig. 3 | Acta Neuropathologica Communications

Fig. 3

From: Targeted deletions of complement lectin pathway genes improve outcome in traumatic brain injury, with MASP-2 playing a major role

Fig. 3

Histological analysis of lesion at 6 weeks after TBI in WT and MASP-2−/− mice. a Representative image of Cresyl violet staining in ipsilateral and contralateral hemisphere. The lesion volume was evaluated based on the different extents of the ipsilateral and contralateral hemispheres, outlined in blue. Scale bar 1 mm. b Representative images of quantified sections for lesion volume at 6 weeks after TBI in WT and MASP-2−/− mice. Distance from bregma in mm is indicated. c Quantification of the lesion showed no differences between WT and MASP-2−/− mice 6 weeks after TBI. The data is shown as a scatter dot plot, line at mean ± SEM (n = 6); Mann–Whitney test. d Anatomical location of area of interest (red box). e Positioning of the cortical region of interest (blue outline) for calculating neuronal cell viability, traced at a distance of 350 μm from the contusion edge. Scale bar 350 μm. f 20 × high magnified fields of view showing a higher presence of neurons in MASP-2−/− than WT mice. Scale bars 20 μm. g Quantification of neuronal density in the region of interest. MASP-2−/− mice had higher neuronal density in the ipsilateral cortex than WT mice. The data is shown as a scatter dot plot, line at mean ± SEM (n = 5–6); Two-way Anova followed by Sidak’s post hoc test, *p < 0.05 compared with ipsi WT

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