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Fig. 5 | Acta Neuropathologica Communications

Fig. 5

From: Network analysis of the progranulin-deficient mouse brain proteome reveals pathogenic mechanisms shared in human frontotemporal dementia caused by GRN mutations

Fig. 5

Galectin-3 levels are robustly increased with age in Grn−/− mouse brain. a, b Representative immunoblot (left) and quantification (right) of galectin-3 levels in the brain lysates of 3- and 18-month-old Grn+/+ mice and age-matched Grn−/− mice (n = 4 mice each group). Band values were normalized by total protein values and analyzed by two-way ANOVA. c Levels of mouse brain galectin-3 (ng/mg of protein) in lysates were quantified by ELISA. Galectin-3 levels were normalized to total protein added to assay and results analyzed by two-way ANOVA. d Galectin-3 protein measured in 19-month-old Grn+/+ and Grn−/− mice plasma samples by ELISA. Data analyzed using unpaired t-test. e Brain sections from Grn+/+, Grn+/−, Grn−/− mice at 3-, 12- and 24-months of age were immunostained with galectin-3 antibody. Representative images of thalamus from each genotype are shown. f 19-month-old Grn+/+ and age-matched Grn−/− mice brain coronal sections were stained with anti-GPNMB antibody and images from multiple regions (cortex (I, VII), hippocampus (II, VIII), corpus callosum (III, IX), striatum (IV, X), thalamus (V, XI) and hypothalamus (VI, XII)) are shown. Scale bars (1 nm to 100 µm) are labeled in images and quantitative data shown as mean ± SEM, p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001

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