Fig. 1

LM11A-31 treatment modulates mechanisms regulating tau cleavage and phosphorylation. a–f Western blots of hippocampal extracts (representative examples shown) were quantitated by determining ratios of cleaved to non-cleaved fragments or actin, or ratios of phospho (p)-protein over total protein and normalized to the mean of Ntg-vehicle mice a ratio of ~ 145 kDa α-fodrin calpain cleavage fragments to actin. b Ratio of CDK5 regulatory subunit p25 (active, calpain cleavage product) to full length p35 inactive subunit. c p-JNK^T183/Y185 to total JNK. d p-GSK3β^S9 to total GSK3β, e p-ERK^T202/Y204 to total ERK. f p-cofilin^S3 to total cofilin. p-values for the indicated comparisons are shown and statistical significance was determined using an ANOVA with post hoc Sidak’s multiple comparisons test; n = 6–11 mice per group, with two or three independent western blots averaged per animal