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Fig. 2 | Acta Neuropathologica Communications

Fig. 2

From: Aggregate-selective antibody attenuates seeded aggregation but not spontaneously evolving disease in SOD1 ALS model mice

Fig. 2

Mouse monoclonal antibodies bind spinal cord derived hSOD1 aggregates. a Immunohistochemical staining of proteinase-resistant hSOD1 aggregates in the ventral horn of spinal cord sections from C57BL/6J non-Tg control (Wt); 200-day-old pre-symptomatic hSOD1G85R Tg; and 400-day terminal stage Tg hSOD1G85R mice, using mAbs raised against the aa 57–72 peptide (hSOD157–72) or aa 131–153 (hSOD1131–153). Scale bar = 40 μm. b Dot blot of filter-captured aggregates from spinal cords of end-stage disease hSOD1G85R and hSOD1G93A Tg mice, probed with the indicated αSOD1 mAbs. Polyclonal rabbit antibodies raised against the same peptides (rbAb hSOD157–72; rbAb hSOD1131–153) were included as positive controls. c Graph represents relative staining intensities of the hSOD1G85R aggregates. To assess aggregate/mAb complex stability, filters were either washed for 1 h at 23 °C or exposed to extended 24 h washing at 23 °C or 37 °C (n = 3 for each condition). Data presented as mean ± SD

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