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Fig. 6 | Acta Neuropathologica Communications

Fig. 6

From: Activation of Toll-like receptor 5 in microglia modulates their function and triggers neuronal injury

Fig. 6

Intrathecal flagellin triggers neuronal injury in the cerebral cortex. 1 μg of flagellin (FLA) was injected intrathecally into C57BL/6 mice (n = 4), while PBS served as control (n = 4). a After 3 d, brain sections were stained with NeuN antibody and DAPI, and representative images are shown (scale bar, 50 μm). b The density of NeuN-positive cells in the cerebral cortex was assessed. c Sections were stained with active caspase-3 antibody (images are not shown), and caspase-3-positive cells in the cerebral cortex were quantified. d Sections were immunostained with Iba1 antibody and DAPI, and e Iba1-positive cells in the cerebral cortex were quantified. Results are presented as mean ± SEM. Data were analyzed by Student’s t-test and provided indicated P values

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