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Fig. 4 | Acta Neuropathologica Communications

Fig. 4

From: Activation of Toll-like receptor 5 in microglia modulates their function and triggers neuronal injury

Fig. 4

Flagellin-induced microglial phagocytosis is mediated by TLR5 and the PI3K/PDK1/Akt/mTOR pathway. a Using FACS-based phagocytosis assay incorporation of Alexa Fluor 405-labelled beads into microglia was quantified. The image shows representative histograms, in which peaks represent the number of microglia that phagocytosed none or one to four beads in the absence (control, black) and presence of flagellin (FLA, 100 ng/ml, gray). b The phagocytic index derived from the data shown in (a) was determined and normalized to control. In addition, FLA-treated microglia in the presence of LY294002 (50 μM), Wortmannin (1 μM), rapamycin (100 μM), or anti-mTLR5-IgG (1 μg/ml) were tested (n = 4). DMSO-containing DMEM served as control, while FLA was solved in DMSO-containing DMEM. Results are expressed as mean ± SEM. Data were analyzed by one-way ANOVA followed by Tukey’s post hoc test. **P < 0.01 vs. control; ##P < 0.01 vs. FLA

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