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Fig. 5 | Acta Neuropathologica Communications

Fig. 5

From: Co-expression of C9orf72 related dipeptide-repeats over 1000 repeat units reveals age- and combination-specific phenotypic profiles in Drosophila

Fig. 5

Histological analysis of Drosophila adult brains pan-neuronally expressing DPRs. a Histological sections of adult Drosophila brains at 28 days post-eclosion reveals vacuolar holes (examples labelled with arrows), characteristic of neurodegeneration in the Drosophila central nervous system. Scale bars 100 μm. b at 28 days post-eclosion pan-neuronal expression of DPRs results in a clear increase in the number of cells positive for the apoptotic marker cleaved caspase 3 (CC3) (arrows show examples), compared to age matched control (mCD8-GFP) flies. Scale bars 50 μm. c, d Quantification of the number of vacuoles > 5 μm (c) and > 10 μm (d) reveals a significant increase in the number of vacuoles in flies 28 days post-eclosion pan-neuronally expressing (nSyb-Gal4) UAS-AP1000 and UAS-GR1000, compared to age-matched controls (ANOVA with post hoc Dunnett’s multiple comparison to wild type controls ****p < .0001,; ***p < .001 ; *p < .05) n = 3 brains per genotype. e Quantification of the number of CC3 positive cells within central brain of adult Drosophila pan-neuronally expressing (nSyb-Gal4) DPRs at 28 days post-eclosion. (ANOVA with post hoc Dunnett’s multiple comparison to wild type controls ****p < .0001; ***p < .001; **p < .01). n is shown on bars

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